NylB'-SCY_pET21b
(Plasmid #215417)

• Purpose: Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli
• Depositing Lab: Gregg Beckham
• Publication: Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5.

Backbone

• Vector backbone: pET21b
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NylB'-SCY
• Alt name: Nylon dimer hydrolase from Flavobacterium sp.
• Species: Flavobacterium sp.
• Mutation: R187S/F264C/D370Y

Cloning Information

• Cloning method: Gene Synthesis

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene from nylon dimer hydrolase described in Ohki, T. et al. Two alternative modes for optimizing nylon-6 byproduct hydrolytic activity from a carboxylesterase with a beta-lactamase fold: X-ray crystallographic analysis of directly evolved 6-aminohexanoate-dimer hydrolase. Protein Sci. 18, 1662-1673 (2009).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry