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Addgene

HotPETase_pET21b
(Plasmid #215407)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215407 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HotPETase
  • Alt name
    PETase from Ideonella Sakiensis
  • Species
    Ideonella Sakiensis
  • Mutation
    S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N233C/S282C/R90T/Q182M/N212K/R224L/S58A/S61V/K95N/M154G/N241C/K252M/T270Q

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene from PETase described in Bell, E. L. et al. Directed evolution of an efficient and thermostable PET depolymerase. Nat. Catal. 5, 673-681 (2022).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HotPETase_pET21b was a gift from Gregg Beckham (Addgene plasmid # 215407 ; http://n2t.net/addgene:215407 ; RRID:Addgene_215407)

  • For your References section:

    Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization. Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT. Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5. 10.1038/s41467-024-45523-5 PubMed 38336849
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