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AfNitB_pET29b
(Plasmid #215401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AfNitB
  • Alt name
    Nitrilase from Acidovorax fascilis
  • Species
    Acidovorax fascilis

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene from nitrilase described in Tateno, T., Tokuda, J. & Kai, K. Mutant nitrile hydratase, nucleic acid encoding the mutant nitrile hydratase, expression vector and transformant containing the nucleic acid, production method for muntant nitrile hydratase, and production method for amide compound. (2018).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AfNitB_pET29b was a gift from Gregg Beckham (Addgene plasmid # 215401 ; http://n2t.net/addgene:215401 ; RRID:Addgene_215401)

  • For your References section:

    Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization. Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT. Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5. 10.1038/s41467-024-45523-5 PubMed 38336849
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