Skip to main content
Addgene

pRSET-Cm-CspA1
(Plasmid #215398)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215398 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRSET
  • Backbone size w/o insert (bp) 4777
  • Total vector size (bp) 3161
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protein Expression Induced at 28°C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cspA1
  • Species
    Clavibacter michiganensis
  • Insert Size (bp)
    204
  • Mutation
    Codon-optimized for E. coli BL21(DE3)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6xHis Tag (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGGGACAAGTTTGTACAAAAAAGCAGGCT
  • 3′ sequencing primer GGGGACCACTTTGTACAAGAAAGCTGGGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://pubmed.ncbi.nlm.nih.gov/37790530/ for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET-Cm-CspA1 was a gift from Gitta Coaker (Addgene plasmid # 215398 ; http://n2t.net/addgene:215398 ; RRID:Addgene_215398)

  • For your References section:

    Natural variation of immune epitopes reveals intrabacterial antagonism. Stevens DM, Moreno-Perez A, Weisberg AJ, Ramsing C, Fliegmann J, Zhang N, Madrigal M, Martin G, Steinbrenner A, Felix G, Coaker G. Proc Natl Acad Sci U S A. 2024 Jun 4;121(23):e2319499121. doi: 10.1073/pnas.2319499121. Epub 2024 May 30. 10.1073/pnas.2319499121 PubMed 38814867
Related items: