FLAG-Ago2
(Plasmid #21538)

• Depositing Lab: Edward Chan
• Publication: Lian et al RNA. 2009 May . 15(5):804-13.

Backbone

• Vector backbone: p3XFLAG-myc-CMV-24
• Backbone size w/o insert (bp): 4752
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ago2
• Alt name: EIF2C2
• Species: H. sapiens (human)
• Insert Size (bp): 2600
• Mutation: The first 7aa of this Ago2 protein are different from the NCBI website Ref sequ
• GenBank ID: NM_012154
• Entrez Gene: AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
• Tag / Fusion Protein:
  • 3XFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV24sp

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original Ago2 clone from Dr. Tom Hobman at Univ. of Calgary
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The first 7aa of our Ago2 protein are different from NCBI website Ref seq. Addgene NGS results identified an R372M variant in Ago2.