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Addgene

NHA-Ago4
(Plasmid #21537)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCl-neo_NHA
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ago4
  • Alt name
    EIF2C4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • GenBank ID
    NM_017629
  • Entrez Gene
    AGO4 (a.k.a. EIF2C4)
  • Tag / Fusion Protein
    • NHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Dra I (destroyed during cloning)
  • 3′ cloning site EcoR V (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NHA-Ago4 was a gift from Edward Chan (Addgene plasmid # 21537 ; http://n2t.net/addgene:21537 ; RRID:Addgene_21537)

  • For your References section:

    The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK. RNA. 2009 May . 15(5):804-13. 10.1261/rna.1229409 PubMed 19324964
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