Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

Actin Binding Domain Tension Sensor
(Plasmid #215368)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215368 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5397
  • Total vector size (bp) 7263
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Actin Binding Domain Tension Sensor
  • Alt name
    ADBTS
  • Species
    Synthetic
  • Insert Size (bp)
    1866
  • Promoter CMV

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dyche Mullins (F-tractin and Linker 1 from Addgene plasmid # 58473) and Martin Schwartz (Original TSMod from Addgene plasmid # 26021).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

F-tractin is based on the rat neuronal inositol 1,4,5-triphosphate 3-kinase A (ITPKA) actin-binding domain.

Please visit https://www.biorxiv.org/content/10.1101/2024.01.10.575065v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Actin Binding Domain Tension Sensor was a gift from Brenton Hoffman (Addgene plasmid # 215368 ; http://n2t.net/addgene:215368 ; RRID:Addgene_215368)
  • For your References section:

    Detection of fluorescent protein mechanical switching in cellulo. Shoyer TC, Collins KL, Ham TR, Blanchard AT, Malavade JN, Johns BA, West JL, Hoffman BD. Cell Rep Methods. 2024 Jul 15;4(7):100815. doi: 10.1016/j.crmeth.2024.100815. Epub 2024 Jul 9. 10.1016/j.crmeth.2024.100815 PubMed 38986612