Actin Binding Domain Tension Sensor
(Plasmid #215368)

• Purpose: Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.
• Depositing Lab: Brenton Hoffman
• Publication: Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):100815. doi: 10.1016/j.crmeth.2024.100815. Epub 2024 Jul 9.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5397
• Total vector size (bp): 7263
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Actin Binding Domain Tension Sensor
• Alt name: ADBTS
• Species: Synthetic
• Insert Size (bp): 1866
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pcdna3-1_abdts_withoriginaltsmod_mtfp1_mvenus.gb
• A portion of this plasmid was derived from a plasmid made by: Dyche Mullins (F-tractin and Linker 1 from Addgene plasmid # 58473) and Martin Schwartz (Original TSMod from Addgene plasmid # 26021).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

F-tractin is based on the rat neuronal inositol 1,4,5-triphosphate 3-kinase A (ITPKA) actin-binding domain.

Please visit https://www.biorxiv.org/content/10.1101/2024.01.10.575065v1 for bioRxiv preprint.