pMRX-IB-TMEM192-mKeima
(Plasmid #215359)

• Purpose: Probe to evaluate lysophagic activity
• Depositing Lab: Tamotsu Yoshimori
• Publication: Shima et al J Cell Biol. 2023 Dec 4;222(12):e202204048. doi: 10.1083/jcb.202204048. Epub 2023 Oct 6.

Backbone

• Vector backbone: pMRX-IB
• Backbone manufacturer: Dr. Yamaoka (Tokyo Medical and Dental University, Tokyo, Japan)
• Backbone size w/o insert (bp): 5867
• Total vector size (bp): 7400
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: transmembrane protein 192
• Species: H. sapiens (human)
• Insert Size (bp): 1533
• GenBank ID: NM_001100389.1
• Entrez Gene: TMEM192
• Tag / Fusion Protein:
  • mKeima (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GGTGGTACGGGAATTATGGCGGCGGGGGGCAGG
• 3′ sequencing primer: GAAACCGTTGCGGCCTTAACCGAGCAAAGAGTGG

Resource Information

• Supplemental Documents:
  • pMRXib-TMEM192-mKeima.gbk
  • pMRXib-TMEM192-mKeima.gbk
• A portion of this plasmid was derived from a plasmid made by: The genes encoding mKeima and TMEM192 were obtained through Addgene plasmids #54597 and #102930, respectively.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please acknowledge Dr. Yamaoka (Tokyo Medical and Dental University, Tokyo, Japan) as the provider of the pMRX-IB vector and, if applicable, cite the original article (PMID: 12840022, DOI: 10.1074/jbc.M304266200) in your future publications.