V. natriegens NC1
(Bacterial strain
#215355)
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PurposeVibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 215355 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonena
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Selectable markers2ug/mL chloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)This is a strain.
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Growth instructionsSee depositor comments.
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Copy numberUnknown
Gene/Insert
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Gene/Insert namena
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genotype: ATCC14048 + ∆dns + camR + tfoX + lacI (nonfunctional).
Supporting References: Chromosome 1 sequence: https://benchling.com/s/seq-9rfOeT70F35Li9CNjCnA?m=slm-n4lGsmu5T0KaunpiH620.
Culture in LBv2 or LBv3 broth with 2ug/mL chloramphenicol.
Recipe for LBv2: https://www.protocols.io/view/growth-media-for-v-natriegens-kqdg349j7l25/v1.
Please visit https://www.biorxiv.org/content/10.1101/2023.08.11.553013v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
V. natriegens NC1 was a gift from Buz Barstow (Addgene plasmid # 215355)