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Addgene

T. aq RNA Polymerase
(Plasmid #215349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Total vector size (bp) 14900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNA polymerase
  • Alt name
    RNAP
  • Species
    Thermus aquaticus
  • Insert Size (bp)
    9786
  • GenBank ID
    CP010822.1 Y19222.2
  • Tag / Fusion Protein
    • 6x His (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Konstantin Kuznedelov, Konstantin Severinov

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.12.05.570056/ for bioRxiv preprint.

Article references for protein purification:
(1) Kuznedelov, K., Minakhin, L., & Severinov, K. (2003). Preparation and characterization of recombinant Thermus aquaticus RNA polymerase. Methods in enzymology, 370, 94–108. https://doi.org/10.1016/S0076-6879(03)70009-3.
(2) Kuznedelov, K., Lamour, V., Patikoglou, G., Chlenov, M., Darst, S. A., & Severinov, K. (2006). Recombinant Thermus aquaticus RNA polymerase for structural studies. Journal of Molecular Biology, 359(1), 110–121. https://doi.org/10.1016/j.jmb.2006.03.009.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T. aq RNA Polymerase was a gift from Julius Lucks (Addgene plasmid # 215349 ; http://n2t.net/addgene:215349 ; RRID:Addgene_215349)

  • For your References section:

    The effect of pseudoknot base pairing on cotranscriptional structural switching of the fluoride riboswitch. Hertz LM, White EN, Kuznedelov K, Cheng L, Yu AM, Kakkaramadam R, Severinov K, Chen A, Lucks JB. Nucleic Acids Res. 2024 May 8;52(8):4466-4482. doi: 10.1093/nar/gkae231. 10.1093/nar/gkae231 PubMed 38567721
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