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Addgene

pYZ1389
(Plasmid #215348)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215348 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGCC4
  • Backbone size w/o insert (bp) 9268
  • Total vector size (bp) 12960
  • Vector type
    CRISPR ; Nla i Delivery Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The plasmid can be maintained using either Kanamycin or Erythromycin separately.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nla CRISPRi Genes (Cas5, Cas8c, Cas7)
  • Species
    Neisseria lactamica

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Yan Zhang -- University of Michigan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYZ1389 was a gift from Hank Seifert (Addgene plasmid # 215348 ; http://n2t.net/addgene:215348 ; RRID:Addgene_215348)

  • For your References section:

    Development and implementation of a Type I-C CRISPR-based programmable repression system for Neisseria gonorrhoeae. Geslewitz WE, Cardenas A, Zhou X, Zhang Y, Criss AK, Seifert HS. mBio. 2023 Dec 21:e0302523. doi: 10.1128/mbio.03025-23. 10.1128/mbio.03025-23 PubMed 38126782
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