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Addgene

Spacer Switch Plasmid
(Plasmid #215346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist Kan Medium Copy
  • Backbone manufacturer
    Twist
  • Backbone size w/o insert (bp) 2105
  • Total vector size (bp) 4305
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OpaD spacer; Chloramphenicol Resistance Cassette
  • Species
    Neisseria gonorrhoeae
  • Insert Size (bp)
    2200

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Spacer Switch Plasmid was a gift from Hank Seifert (Addgene plasmid # 215346 ; http://n2t.net/addgene:215346 ; RRID:Addgene_215346)

  • For your References section:

    Development and implementation of a Type I-C CRISPR-based programmable repression system for Neisseria gonorrhoeae. Geslewitz WE, Cardenas A, Zhou X, Zhang Y, Criss AK, Seifert HS. mBio. 2023 Dec 21:e0302523. doi: 10.1128/mbio.03025-23. 10.1128/mbio.03025-23 PubMed 38126782
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