pGL004
(Plasmid #215336)

• Purpose: (Empty Backbone) amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)
• Depositing Lab: Gene-Wei Li
• Publication: A historical sequence deletion in a commonly used Bacillus subtilis chromosome integration vector generates undetected loss-of-function mutations (unpublished)

Backbone

• Vector backbone: pDR111
• Backbone size (bp): 7853
• Modifications to backbone: Removed 156 bp following the amyE back homology arm
• Vector type: Bacterial Expression
• Promoter: hyper-pSpank

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pGL004.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A copy of pDR111 was obtained from the Grossman lab at MIT for subsequent modification to generate pGL004.