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pKb-TnpB1
(Plasmid #215333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRGEB32
  • Backbone manufacturer
    Yinong Yang
  • Backbone size w/o insert (bp) 11743
  • Total vector size (bp) 13060
  • Modifications to backbone
    1. Replaced gRNA scaffold with reRNA-gRNA cloning site-HDV ribozyme. 2. Replaced Cas9 with rice codon optimized TnpB nuclease.
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rice codon optimized ISDra2 TnpB
  • Species
    Deinococcus radiodurans
  • Insert Size (bp)
    1224
  • Promoter Rice ubiquitin promoter for TnpB and OsU3 promoter for reRNA+guide RNA expression
  • Tag / Fusion Protein
    • Not applicable

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKb-TnpB1 was a gift from Kutubuddin Molla (Addgene plasmid # 215333 ; http://n2t.net/addgene:215333 ; RRID:Addgene_215333)

  • For your References section:

    A miniature alternative to Cas9 and Cas12: Transposon-associated TnpB mediates targeted genome editing in plants. Karmakar S, Panda D, Panda S, Dash M, Saha R, Das P, Avinash SP, Shih J, Yang Y, Nayak AK, Baig MJ, Molla KA. Plant Biotechnol J. 2024 Jun 28. doi: 10.1111/pbi.14416. 10.1111/pbi.14416 PubMed 38940895
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