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Addgene

131-2a_LC
(Plasmid #215331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 5038
  • Total vector size (bp) 5752
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    131-2a_LC
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    714
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer GAAGAGGAAGAAAGGGAAAC
  • 3′ sequencing primer CTGTAATTGACAGCCTTGCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.04.631317 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    131-2a_LC was a gift from Joost Snijder (Addgene plasmid # 215331 ; http://n2t.net/addgene:215331 ; RRID:Addgene_215331)

  • For your References section:

    Structural basis for postfusion-specific binding to Respiratory Syncytial Virus F protein by the canonical antigenic site I antibody 131-2a. Peng W, Šiborová M, Wu X, Du W, Schulte D, Pronker MF, de Haan CAM, Snijder J. bioRxiv 2025.01.04.631317 10.1101/2025.01.04.631317
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