pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2
(Plasmid #215327)

• Purpose: Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.
• Depositing Lab: Rob Hynds
• Publication: Orr et al Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L587-L599. doi: 10.1152/ajplung.00047.2024. Epub 2024 Aug 13.

Backbone

• Vector backbone: pCCL-NoPromoter-FLuc-CMV-RLuc-dsRed2
• Backbone size w/o insert (bp): 10530
• Total vector size (bp): 11589
• Vector type: Mammalian Expression, Lentiviral, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MUC5AC Promoter
• Species: H. sapiens (human)
• Insert Size (bp): 1059
• Promoter: MUC5AC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: AATTGTCTCGAGACTCACTGGGACCTTTCTGT
• 3′ sequencing primer: AATTCAGGATCCCAGCTTCCTCCGGCCAACAC

Resource Information

• Supplemental Documents:
  • pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.09.579635 for bioRxiv preprint.