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Addgene

pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2
(Plasmid #215327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCCL-NoPromoter-FLuc-CMV-RLuc-dsRed2
  • Backbone size w/o insert (bp) 10530
  • Total vector size (bp) 11589
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MUC5AC Promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1059
  • Promoter MUC5AC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AATTGTCTCGAGACTCACTGGGACCTTTCTGT
  • 3′ sequencing primer AATTCAGGATCCCAGCTTCCTCCGGCCAACAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.09.579635 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2 was a gift from Rob Hynds (Addgene plasmid # 215327 ; http://n2t.net/addgene:215327 ; RRID:Addgene_215327)

  • For your References section:

    A lentiviral toolkit to monitor airway epithelial cell differentiation using bioluminescence. Orr JC, Laali A, Durrenberger PF, Lazarus KA, El Mdawar M, Janes SM, Hynds RE. bioRxiv 2024.02.09.579635 10.1101/2024.02.09.579635
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