pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2
(Plasmid #215326)

• Purpose: Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.
• Depositing Lab: Rob Hynds
• Publication: Orr et al Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L587-L599. doi: 10.1152/ajplung.00047.2024. Epub 2024 Aug 13.

Backbone

• Vector backbone: pCCL-NoPromoter-FLuc-CMV-RLuc-dsRed2
• Backbone size w/o insert (bp): 10546
• Total vector size (bp): 12189
• Vector type: Mammalian Expression, Lentiviral, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dNTP63 Promoter
• Alt name: TP63 Promoter
• Alt name: P63 Promoter
• Species: H. sapiens (human)
• Insert Size (bp): 1659
• Promoter: dNTP63

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: AATGGTCTCGAGGCTGTCAGTAGGTGTAGAATTTAG
• 3′ sequencing primer: AATGGAGGATCCCAATATGAATCTACTTAAGAAGATAACAGA

Resource Information

• Supplemental Documents:
  • pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.09.579635 for bioRxiv preprint.