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CDKN1A (p21) targeting gRNA
(Plasmid #215319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px458 (Plasmid #48138)
  • Modifications to backbone
    Targeting CDKN1A (p21).
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hSpCas9
  • Alt name
    SpCas9-2A-GFP
  • Alt name
    Cas9
  • gRNA/shRNA sequence
    GATGTCCGTCAGAACCCATG
  • Species
    Synthetic
  • Promoter Cbh
  • Tags / Fusion Proteins
    • 3XFLAG (N terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CDKN1A (p21) targeting gRNA was a gift from Michele Pagano (Addgene plasmid # 215319 ; http://n2t.net/addgene:215319 ; RRID:Addgene_215319)

  • For your References section:

    CDK-independent role of D-type cyclins in regulating DNA mismatch repair. Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M. Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 10.1016/j.molcel.2024.02.010. 10.1016/j.molcel.2024.02.010 PubMed 38458201
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