pMVV103
(Plasmid #215309)

• Purpose: Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.
• Depositing Lab: Brian Pfleger
• Publication: Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-3634. doi: 10.1021/acssynbio.3c00414. Epub 2023 Nov 21.

Backbone

• Vector backbone: BBR1
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4749
• Modifications to backbone: Constitutive TetR expression
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sfGFP
• Species: Synthetic
• Insert Size (bp): 711
• Promoter: Ptet
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: na

Resource Information

• Supplemental Documents:
  • pMVV103 (BBR1 TetR PTet-sfGFP).gb
• A portion of this plasmid was derived from a plasmid made by: Optimized induction cassettes were cloned from the Marionette sensor collection into broad host origin BBR1: https://www.addgene.org/kits/marionette-sensor-collection/ https://doi.org/10.1038/s41589-018-0168-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry