pX552 EF1a DIO Chronos GFP_Vgat gRNA
(Plasmid #215277)

• Purpose: Expresses Vgat gRNA in a Cre dependent Chronos GFP vector
• Depositing Lab: Bryan Copits
• Publication: Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.2024. doi: 10.1523/ENEURO.0438-23.2024. Print 2024 Jul.

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Backbone

• Vector backbone: pX552
• Total vector size (bp): 7143
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vgat gRNA
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SapI (destroyed during cloning)
• 3′ cloning site: SapI (destroyed during cloning)
• 5′ sequencing primer: CAAGGCTGTTAGAGAGATAATTGGA
• 3′ sequencing primer: GATGCTTGGATCAAAATAGGAAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.10.561249 for bioRxiv preprint.