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Addgene

GB4003
(Plasmid #215248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDGB3_omega1
  • Backbone manufacturer
    self-made; derived from pCambia1302 generated at the Cambia Institute
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIF6-PhyB:VP16
  • Species
    Synthetic
  • Mutation
    V234L in PIF6- please see dep. sequence, BsaI and BsmBI sites removed

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms V234L mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GB4003 was a gift from Diego Orzaez (Addgene plasmid # 215248 ; http://n2t.net/addgene:215248 ; RRID:Addgene_215248)

  • For your References section:

    A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology. Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D. Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. Epub 2023 Oct 26. 10.1111/pbi.14146 PubMed 37882352
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