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Addgene

GB3774
(Plasmid #215247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUPD2
  • Backbone manufacturer
    self-made; derived from the BioBrick assembly plasmid pSB1C3
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CPH
  • Species
    N. nambi
  • Mutation
    BsaI and BsmBI sites removed

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GB3774 was a gift from Diego Orzaez (Addgene plasmid # 215247 ; http://n2t.net/addgene:215247 ; RRID:Addgene_215247)

  • For your References section:

    A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology. Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D. Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. Epub 2023 Oct 26. 10.1111/pbi.14146 PubMed 37882352
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