CCND1 targeting gRNA
(Plasmid #215153)

• Purpose: Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.
• Depositing Lab: Michele Pagano
• Publication: Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 10.1016/j.molcel.2024.02.010.

Backbone

• Vector backbone: px458 (Plasmid #48138)
• Modifications to backbone: Targeting CCND1.
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hSpCas9
• Alt name: SpCas9-2A-GFP
• Alt name: Cas9
• gRNA/shRNA sequence: CGCGTACCCCGATGCCAACC
• Species: Synthetic
• Promoter: Cbh
• Tags / Fusion Proteins:
  • 3XFLAG (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: hU6-F

Resource Information

• Supplemental Documents:
  • CCND1 targeting gRNA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry