mCherry-POLE1
(Plasmid #215140)

• Purpose: Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.
• Depositing Lab: Michele Pagano
• Publication: Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 10.1016/j.molcel.2024.02.010.

Backbone

• Vector backbone: pRetroQ-C1
• Backbone manufacturer: Takara Bio
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: POLE1
• Alt name: POLE
• Species: H. sapiens (human)
• Mutation: Silent mutations in amino acid 80-86 and 211-217 (to resist siRNA).
• Entrez Gene: POLE (a.k.a. CRCS12, FILS, IMAGEI, POLE1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry_FWR (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: mCherry_FWR
• 3′ sequencing primer: PGK_REV

Resource Information

• Supplemental Documents:
  • mCherry-POLE1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry