FLAG-D1-IRES-SS-D2-IRES-HA-D3
(Plasmid #215119)

• Purpose: Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.
• Depositing Lab: Michele Pagano
• Publication: Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 10.1016/j.molcel.2024.02.010.

Backbone

• Vector backbone: pLV.puro
• Backbone manufacturer: Vectorbuilder
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CCND1
• Species: H. sapiens (human)
• Entrez Gene: CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
• Promoter: TREG3
• Tag / Fusion Protein:
  • FLAG-tag (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: TREG3

Gene/Insert 2

• Gene/Insert name: CCND2
• Species: H. sapiens (human)
• Entrez Gene: CCND2 (a.k.a. KIAK0002, MPPH3)
• Promoter: IRES
• Tag / Fusion Protein:
  • SS (STREP-STREP) (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: STREP-FWR

Gene/Insert 3

• Gene/Insert name: CCND3
• Species: H. sapiens (human)
• Entrez Gene: CCND3
• Promoter: IRES
• Tag / Fusion Protein:
  • HA-tag (N terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: HA-FWR

Resource Information

• Supplemental Documents:
  • tricistronic-expression-vector-2nd-gen.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry