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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21509 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR P2R-P3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2639
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Vector typeMultiSite Gateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5a
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Copy numberUnknown
Gene/Insert
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Gene/Insert name(Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
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SpeciesC. elegans (nematode)
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Insert Size (bp)1223
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attP2R (destroyed during cloning)
- 3′ cloning site attP3 (destroyed during cloning)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R/ T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe tbb-2 3'UTR sequence was taken from pCM1.36.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJA256 was a gift from Julie Ahringer (Addgene plasmid # 21509 ; http://n2t.net/addgene:21509 ; RRID:Addgene_21509) -
For your References section:
MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline. Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J. PLoS One. 2011;6(5):e20082. Epub 2011 May 26. 10.1371/journal.pone.0020082 PubMed 21637852