Skip to main content
Addgene

mCherry-Tsg101
(Plasmid #21505)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21505 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    unknown
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tsg101
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1173
  • Mutation
    none
  • Entrez Gene
    TSG101 (a.k.a. TSG10, VPS23)
  • Tag / Fusion Protein
    • Cherry*

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note that despite the plasmid name, Addgene's sequencing result suggests that the fluor is RFP, not mCherry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Tsg101 was a gift from James Hurley (Addgene plasmid # 21505 ; http://n2t.net/addgene:21505 ; RRID:Addgene_21505)

  • For your References section:

    Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55. Lee HH, Elia N, Ghirlando R, Lippincott-Schwartz J, Hurley JH. Science. 2008 Oct 24. 322(5901):576-80. 10.1126/science.1162042 PubMed 18948538
Related items:
Commonly requested with: