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Addgene

mCherry-hALIX
(Plasmid #21504)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21504 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALIX
  • Alt name
    PDCD6IP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2607
  • Mutation
    none
  • Entrez Gene
    PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
  • Tag / Fusion Protein
    • Cherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-hALIX was a gift from James Hurley (Addgene plasmid # 21504 ; http://n2t.net/addgene:21504 ; RRID:Addgene_21504)

  • For your References section:

    Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55. Lee HH, Elia N, Ghirlando R, Lippincott-Schwartz J, Hurley JH. Science. 2008 Oct 24. 322(5901):576-80. 10.1126/science.1162042 PubMed 18948538
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