pGST2-ALIX (795-811)
(Plasmid
#21502)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGST2
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameALIX
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Alt namePDCD6IP
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SpeciesH. sapiens (human)
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Insert Size (bp)54
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Mutationnone
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Entrez GenePDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GTA TAT AGC ATG GCC TTT GCA GGG CTG G (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGST2-ALIX (795-811) was a gift from James Hurley (Addgene plasmid # 21502 ; http://n2t.net/addgene:21502 ; RRID:Addgene_21502) -
For your References section:
Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55. Lee HH, Elia N, Ghirlando R, Lippincott-Schwartz J, Hurley JH. Science. 2008 Oct 24. 322(5901):576-80. 10.1126/science.1162042 PubMed 18948538