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Addgene

pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)
(Plasmid #215015)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215015 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHAGE pGK
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin ; mGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAP1LC3B
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
  • Tag / Fusion Protein
    • HaloTag(7)-mGFP (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP) was a gift from Wade Harper (Addgene plasmid # 215015 ; http://n2t.net/addgene:215015 ; RRID:Addgene_215015)