pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)
(Plasmid
#215014)
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PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 215014 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti UBC
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin ; mGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMAP1LC3B
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SpeciesR. norvegicus (rat)
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Entrez GeneMap1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
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Tag
/ Fusion Protein
- HaloTag(7)-mGFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP) was a gift from Wade Harper (Addgene plasmid # 215014 ; http://n2t.net/addgene:215014 ; RRID:Addgene_215014)
