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Addgene

pGST2-CeEP55
(Plasmid #21501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGST2
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CEP55-EABR (ESCRT and ALIX Binding Region) domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    918
  • Mutation
    Q230 (CAG changed to TAA), Q218 (CAA changed to TAA)
  • Entrez Gene
    CEP55 (a.k.a. C10orf3, CT111, MARCH, URCC6)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTA TAT AGC ATG GCC TTT GCA GGG CTG G
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PNC to MGS mut in beginning of protein; author says that this is the plasmid that was used in publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST2-CeEP55 was a gift from James Hurley (Addgene plasmid # 21501 ; http://n2t.net/addgene:21501 ; RRID:Addgene_21501)

  • For your References section:

    Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55. Lee HH, Elia N, Ghirlando R, Lippincott-Schwartz J, Hurley JH. Science. 2008 Oct 24. 322(5901):576-80. 10.1126/science.1162042 PubMed 18948538
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