pDONR223 LC3Flux (HaloTag7-mGFP-LC3-STOP)
(Plasmid
#215004)
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PurposeShuttling vector for an autophagy (LC3) reporter
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 215004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR223
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Vector typeGateway
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMAP1LC3B
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SpeciesR. norvegicus (rat)
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Entrez GeneMAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
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Tag
/ Fusion Protein
- HaloTag(7)-mGFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid #184901
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223 LC3Flux (HaloTag7-mGFP-LC3-STOP) was a gift from Wade Harper (Addgene plasmid # 215004 ; http://n2t.net/addgene:215004 ; RRID:Addgene_215004)
