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Addgene

pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
(Plasmid #215002)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215002 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR223
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDEL
  • Species
    Other
  • Tag / Fusion Protein
    • HaloTag(7)-mGFP (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #184904

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP) was a gift from Wade Harper (Addgene plasmid # 215002 ; http://n2t.net/addgene:215002 ; RRID:Addgene_215002)