pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP)
(Plasmid
#215001)
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PurposeShuttling vector for a mitophagy reporter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR223
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Vector typeGateway
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepSu9 (Presequence of Fo-ATPase subunit 9)
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SpeciesOther
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Tag
/ Fusion Protein
- HaloTag(7)-mGFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid #184905
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP) was a gift from Wade Harper (Addgene plasmid # 215001 ; http://n2t.net/addgene:215001 ; RRID:Addgene_215001)