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Addgene

pFBD-ESCRT 0
(Plasmid #21499)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21499 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac Dual
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5238
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pFastBac Dual-His/MBP/Hrs-GST/STAM1
  • Alt name
    Hrs, HGS
  • Alt name
    STAM
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    3531
  • Mutation
    none
  • Entrez Gene
    STAM (a.k.a. STAM-1, STAM1)
  • Tags / Fusion Proteins
    • His-MBP (N terminal on backbone)
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (RF cloning used to insert genes) (not destroyed)
  • 5′ sequencing primer Polyhedrin forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFBD-ESCRT 0 was a gift from James Hurley (Addgene plasmid # 21499 ; http://n2t.net/addgene:21499 ; RRID:Addgene_21499)

  • For your References section:

    Hybrid structural model of the complete human ESCRT-0 complex. Ren X, Kloer DP, Kim YC, Ghirlando R, Saidi LF, Hummer G, Hurley JH. Structure. 2009 Mar 11. 17(3):406-16. 10.1016/j.str.2009.01.012 PubMed 19278655
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