pcDNA3.1-ccdB-TwinStrep
(Plasmid
#214988)
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Purpose(Empty Backbone) Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214988 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- TwinStrep (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- TwinStrep (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Swapped tags from pcDNA3.1-ccdB-3xFLAG-V5 (addgene #87063)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-ccdB-TwinStrep was a gift from Wade Harper (Addgene plasmid # 214988 ; http://n2t.net/addgene:214988 ; RRID:Addgene_214988)
