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Addgene

pKH151_PB_flag-LC3B_wt_IRES-BFP
(Plasmid #214976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggybac
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-LC3B
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKH151_PB_flag-LC3B_wt_IRES-BFP was a gift from Wade Harper (Addgene plasmid # 214976 ; http://n2t.net/addgene:214976 ; RRID:Addgene_214976)

  • For your References section:

    Proteome census upon nutrient stress reveals Golgiphagy membrane receptors. Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, Harper JW. Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6. Epub 2023 Sep 27. 10.1038/s41586-023-06657-6 PubMed 37757899
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