pAAV-hSyn-Cepheid1b-ST-WPRE
(Plasmid
#214967)
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PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214967 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 3619
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCepheid1b-ST
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SpeciesSynthetic
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Insert Size (bp)3611
- Promoter human Synapsin
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-Cepheid1b-ST-WPRE was a gift from Peng Zou (Addgene plasmid # 214967 ; http://n2t.net/addgene:214967 ; RRID:Addgene_214967) -
For your References section:
Bright and sensitive red voltage indicators for imaging action potentials in brain slices and pancreatic islets. Han Y, Yang J, Li Y, Chen Y, Ren H, Ding R, Qian W, Ren K, Xie B, Deng M, Xiao Y, Chu J, Zou P. Sci Adv. 2023 Nov 24;9(47):eadi4208. doi: 10.1126/sciadv.adi4208. Epub 2023 Nov 22. 10.1126/sciadv.adi4208 PubMed 37992174