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pRSFDUET-1_VNp-anti-Muc1 mAb
(Plasmid #214954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDUET-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3829
  • Total vector size (bp) 6228
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VNp-anti-Muc1 mAb heavy chain
  • Insert Size (bp)
    1515
  • Entrez Gene
    MUC1 (a.k.a. ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, Ca15-3, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM)
  • Promoter T7
  • Tags / Fusion Proteins
    • VNp (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    VNp-anti-Muc1 mAb light chain
  • Insert Size (bp)
    783
  • Entrez Gene
    MUC1 (a.k.a. ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, Ca15-3, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM)
  • Promoter T7
  • Tag / Fusion Protein
    • VNp (N terminal on insert)

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.17.589891 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDUET-1_VNp-anti-Muc1 mAb was a gift from Dan Mulvihill (Addgene plasmid # 214954 ; http://n2t.net/addgene:214954 ; RRID:Addgene_214954)

  • For your References section:

    Simple recombinant monoclonal antibody production from Escherichia coli. Baker K, Eastwood TA, Garcia E, Lennon C, Mulvihill DP.. Open Biol. 2025 10.1098/rsob.240229
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