pGST-Vps4
(Plasmid #21495)

• Depositing Lab: James Hurley
• Publication: Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Backbone

• Vector backbone: parallel GST2
• Backbone size w/o insert (bp): 5037
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VPS4
• Alt name: CSC1, END13,
• Alt name: GRD13, VPL4 ,
• Alt name: VPT10, DID6
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1314
• Mutation: none
• Entrez Gene: VPS4 (a.k.a. YPR173C, CSC1, DID6, END13, GRD13, VPL4, VPT10)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: GTA TAT AGC ATG GCC TTT GCA GGG CTG G

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry