Skip to main content
Addgene

His6-pAG-RTase
(Plasmid #214932)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 7495
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
  • Alt name
    pAG-RTase
  • Insert Size (bp)
    2157
  • Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524G, L603W)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6-pAG-RTase was a gift from Chuan He (Addgene plasmid # 214932 ; http://n2t.net/addgene:214932 ; RRID:Addgene_214932)

  • For your References section:

    Profiling of RNA-binding protein binding sites by in situ reverse transcription-based sequencing. Xiao Y, Chen YM, Zou Z, Ye C, Dou X, Wu J, Liu C, Liu S, Yan H, Wang P, Zeng TB, Liu Q, Fei J, Tang W, He C. Nat Methods. 2024 Jan 10. doi: 10.1038/s41592-023-02146-w. 10.1038/s41592-023-02146-w PubMed 38200227
Related items:
Commonly requested with: