pLX304_miRFP670nano-P2A-ERM-gCam6f
(Plasmid
#214927)
-
PurposeExpress miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 214927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLX304
-
Backbone manufacturerlentiviral Gateway destination vector
- Backbone size w/o insert (bp) 7661
- Total vector size (bp) 9543
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemiRFP670nano-P2A-ERM-gCam6f
-
Alt namemiRFP670nano
-
Alt namegCaMP6f
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1881
- Promoter CMVp
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made bypLX304 vector backbone is the gift from Alice Y. Ting (Stanford).
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLX304_miRFP670nano-P2A-ERM-gCam6f was a gift from Xinnan Wang (Addgene plasmid # 214927 ; http://n2t.net/addgene:214927 ; RRID:Addgene_214927) -
For your References section:
A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson's disease. Bharat V, Durairaj AS, Vanhauwaert R, Li L, Muir CM, Chandra S, Kwak CS, Le Guen Y, Nandakishore P, Hsieh CH, Rensi SE, Altman RB, Greicius MD, Feng L, Wang X. Cell Rep. 2023 Dec 26;42(12):113544. doi: 10.1016/j.celrep.2023.113544. Epub 2023 Dec 6. 10.1016/j.celrep.2023.113544 PubMed 38060381