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Addgene

CILK1-E80K
(Plasmid #214910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6-Entry
  • Backbone manufacturer
    Origene
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CILK1
  • Alt name
    ICK
  • Alt name
    MRK
  • Species
    H. sapiens (human)
  • Mutation
    E80K substitution
  • Entrez Gene
    CILK1 (a.k.a. ECO, EJM10, ICK, LCK2, MRK, hICK)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc-FLAG (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CILK1-E80K was a gift from Pavel Krejčí (Addgene plasmid # 214910 ; http://n2t.net/addgene:214910 ; RRID:Addgene_214910)

  • For your References section:

    An inactivating mutation in intestinal cell kinase, ICK, impairs hedgehog signalling and causes short rib-polydactyly syndrome. Paige Taylor S, Kunova Bosakova M, Varecha M, Balek L, Barta T, Trantirek L, Jelinkova I, Duran I, Vesela I, Forlenza KN, Martin JH, Hampl A, Bamshad M, Nickerson D, Jaworski ML, Song J, Ko HW, Cohn DH, Krakow D, Krejci P. Hum Mol Genet. 2016 Sep 15;25(18):3998-4011. doi: 10.1093/hmg/ddw240. Epub 2016 Jul 27. 10.1093/hmg/ddw240 PubMed 27466187
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