pMBP-HIS2-Vps20_deltaC
(Plasmid
#21491)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21491 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHis2
- Backbone size w/o insert (bp) 5549
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVps20 Delta_C
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Alt nameVPS20
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Alt nameCHM6
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Alt nameVPT20
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)510
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Mutationbp 511-663 deleted
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Entrez GeneVPS20 (a.k.a. YMR077C, CHM6, VPL10, VPT20)
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Tags
/ Fusion Proteins
- 6x His (N terminal on backbone)
- MBP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site N/A (RF cloning used) (not destroyed)
- 5′ sequencing primer T7 promotor (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMBP-HIS2-Vps20_deltaC was a gift from James Hurley (Addgene plasmid # 21491 ; http://n2t.net/addgene:21491 ; RRID:Addgene_21491) -
For your References section:
Membrane scission by the ESCRT-III complex. Wollert T, Wunder C, Lippincott-Schwartz J, Hurley JH. Nature. 2009 Mar 12. 458(7235):172-7. 10.1038/nature07836 PubMed 19234443