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Addgene

pcDNA3.1/hFGFR3-K650M-V5
(Plasmid #214909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-V5-HIS-TOPO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGFR3
  • Species
    H. sapiens (human)
  • Mutation
    K650M substitution
  • Entrez Gene
    FGFR3 (a.k.a. ACH, CD333, CEK2, HSFGFR3EX, JTK4)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5/His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/hFGFR3-K650M-V5 was a gift from Pavel Krejčí (Addgene plasmid # 214909 ; http://n2t.net/addgene:214909 ; RRID:Addgene_214909)

  • For your References section:

    Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase. Kunova Bosakova M, Nita A, Gregor T, Varecha M, Gudernova I, Fafilek B, Barta T, Basheer N, Abraham SP, Balek L, Tomanova M, Fialova Kucerova J, Bosak J, Potesil D, Zieba J, Song J, Konik P, Park S, Duran I, Zdrahal Z, Smajs D, Jansen G, Fu Z, Ko HW, Hampl A, Trantirek L, Krakow D, Krejci P. Proc Natl Acad Sci U S A. 2019 Feb 19. pii: 1800338116. doi: 10.1073/pnas.1800338116. 10.1073/pnas.1800338116 PubMed 30782830
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