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TR01F/hFGFR3-G380R-V5
(Plasmid #214908)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214908 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TR01F
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGFR3
  • Species
    H. sapiens (human)
  • Mutation
    G380R substitution
  • Entrez Gene
    FGFR3 (a.k.a. ACH, CD333, CEK2, HSFGFR3EX, JTK4)
  • Promoter truncated CMV promoter
  • Tag / Fusion Protein
    • V5/His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The TR01F plasmid was obtained as a gift from Valeri Mossine (PMID: 24376882).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TR01F/hFGFR3-G380R-V5 was a gift from Pavel Krejčí (Addgene plasmid # 214908 ; http://n2t.net/addgene:214908 ; RRID:Addgene_214908)

  • For your References section:

    An RNA aptamer restores defective bone growth in FGFR3-related skeletal dysplasia in mice. Kimura T, Bosakova M, Nonaka Y, Hruba E, Yasuda K, Futakawa S, Kubota T, Fafilek B, Gregor T, Abraham SP, Gomolkova R, Belaskova S, Pesl M, Csukasi F, Duran I, Fujiwara M, Kavkova M, Zikmund T, Kaiser J, Buchtova M, Krakow D, Nakamura Y, Ozono K, Krejci P. Sci Transl Med. 2021 May 5;13(592):eaba4226. doi: 10.1126/scitranslmed.aba4226. 10.1126/scitranslmed.aba4226 PubMed 33952673
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