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Addgene

pMBP-HIS2-Vps20FL
(Plasmid #21490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHis2
  • Backbone size w/o insert (bp) 5549
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS20
  • Alt name
    CHM6
  • Alt name
    VPT20
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    663
  • Mutation
    none
  • Entrez Gene
    VPS20 (a.k.a. YMR077C, CHM6, VPL10, VPT20)
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • MBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (RF cloning used) (not destroyed)
  • 5′ sequencing primer T7 promotor
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMBP-HIS2-Vps20FL was a gift from James Hurley (Addgene plasmid # 21490 ; http://n2t.net/addgene:21490 ; RRID:Addgene_21490)

  • For your References section:

    Membrane scission by the ESCRT-III complex. Wollert T, Wunder C, Lippincott-Schwartz J, Hurley JH. Nature. 2009 Mar 12. 458(7235):172-7. 10.1038/nature07836 PubMed 19234443
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