pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)
(Plasmid
#214891)
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PurposeExpresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMS27/ pUC19
- Backbone size w/o insert (bp) 4939
- Total vector size (bp) 8013
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGtACR2_mCerulean_bHK_Chrimson
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SpeciesSynthetic
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Insert Size (bp)3074
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Tag
/ Fusion Protein
- mCerulean
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPeter Hegemann
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson) was a gift from Alexander Gottschalk (Addgene plasmid # 214891 ; http://n2t.net/addgene:214891 ; RRID:Addgene_214891) -
For your References section:
All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals. Bergs ACF, Liewald JF, Rodriguez-Rozada S, Liu Q, Wirt C, Bessel A, Zeitzschel N, Durmaz H, Nozownik A, Dill H, Jospin M, Vierock J, Bargmann CI, Hegemann P, Wiegert JS, Gottschalk A. Nat Commun. 2023 Apr 6;14(1):1939. doi: 10.1038/s41467-023-37622-6. 10.1038/s41467-023-37622-6 PubMed 37024493