Skip to main content
Addgene

SB53
(Plasmid #21488)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cited2
  • Alt name
    p35srj (MRG1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1027
  • Mutation
    aa1-65 deleted
  • GenBank ID
    AF109161
  • Entrez Gene
    CITED2 (a.k.a. ASD8, MRG-1, MRG1, P35SRJ, VSD2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ecoRI (not destroyed)
  • 3′ cloning site HincII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HA tag added to 5'end of the cited2 cDNA using a blunt-ha-stop-xho oligo. The insert was ligated into the EcoRI/XhoI sites of pcDNA3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SB53 was a gift from Shoumo Bhattacharya (Addgene plasmid # 21488 ; http://n2t.net/addgene:21488 ; RRID:Addgene_21488)

  • For your References section:

    Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM. Genes Dev. 1999 Jan 1. 13(1):64-75. 10.1101/gad.13.1.64 PubMed 9887100
Related items:
Commonly requested with: